anti olig2 goat igg  (R&D Systems)

Journal: Journal of Neuroscience

Article Title: Neonatal E. Coli Infection Causes Neuro-Behavioral Deficits Associated with Hypomyelination and Neuronal Sequestration of Iron

doi: 10.1523/jneurosci.0708-13.2013

Figure Lengend Snippet: Figure 4. Reduced number of oligodendrocytes in the corpus callosum and motor cortex of juvenile mice exposed to E. coli as neonates.Neonatalmice(P3)wereinjectedsubcutaneouslywitheithersaline(CON)orE.coli(ELI),andbraintissuefrommaleand female mice was collected at P35 and Olig2 (red) and GFAP (green) or Olig2 (red) and NG2 (green) labeling was determined. Representativeimagesof(A)Olig2,(B)Olig2/GFAP,(C)Olig2/NG2fromtheCCofCONandELImiceareshown.Averagenumberof Olig2 /NG2 cells in the (D) MCX, (E) CC, and (F) hippocampus (HPC). Average number of Olig2 /NG2 cells in the (G) MCX, (H)CC,and(I)HPC.BarsrepresentthemeanSEM(n6).Meanswithasteriskaresignificantlydifferentfromcontrols(p 0.05), and means with plus sign tend to be different from controls (p 0.1).

Article Snippet: To double label for Olig2 and GFAP, sections were first labeled for Olig2 (goat, 1:100, R&D Systems; donkey anti-goat 2°) and then labeled for GFAP (rabbit, 1:2000, Dako; goat anti-rabbit 2°).

Techniques: Labeling

Journal: Journal of Neuroscience

Article Title: Neonatal E. Coli Infection Causes Neuro-Behavioral Deficits Associated with Hypomyelination and Neuronal Sequestration of Iron

doi: 10.1523/jneurosci.0708-13.2013

Figure Lengend Snippet: Figure8. ELI-associatedincreaseinL-ferritindidnotcolocalizewithmicroglia,astrocytes,oroligodendrocytes.Neonatalmice (P3)wereinjectedsubcutaneouslywitheithersaline(CON)orE.coli(ELI),andbraintissuefrommaleandfemalemicewascollected at P12 and labeling for Iba-1 (pink), GFAP (white), Olig2 (light blue), and L-ferritin (L-Fer; red). Representative pictures of (A) microglia(Iba-1),(D)astrocytes(GFAP),and(G)oligodendrocytes(Olig2)inthecorpuscallosum(proximaltothehippocampalCA1 region)areshown.QuantificationofimmunopositivegliaintheCC,HP,andCXfor(B)Iba-1,(E)GFAP,and(H)Olig2areprovided. Representative images of colabeling with L-ferritin for (C) Iba-1, (F) GFAP, and (I) Olig2 are also provided. Bars represent the mean SEM (n 4). Means with asterisk are significantly different from controls (p 0.05). Means with plus sign denote a tendency (p 0.1). Scale bar, 100 M.

Article Snippet: To double label for Olig2 and GFAP, sections were first labeled for Olig2 (goat, 1:100, R&D Systems; donkey anti-goat 2°) and then labeled for GFAP (rabbit, 1:2000, Dako; goat anti-rabbit 2°).

Techniques: Labeling

Journal: Nature Protocols

Article Title: Differentiation of human oligodendrocytes from pluripotent stem cells

doi: 10.1038/nprot.2009.186

Figure Lengend Snippet: Figure 3 | Generation of pre-oligodendrocyte progenitor cells (OPCs) and OPCs. The pre-OPCs grow as spheres in suspension at day 35 (a) and express both OLIG2 and NKX2.2 (b). Dissociated OPCs after 14 weeks of human embryonic stem cell (hESC) differentiation exhibit a bipolar morphology (c, arrows), and co-express OLIG2 and platelet-derived growth factor receptor alpha (PDGFRα) (d). At 4 weeks after growing on substrate for differentiation, OPCs become multipolar immature oligodendrocytes (e), and many of these cells express O4 (f). Scale bar, 50 µm. Ho denotes Hoechst 33258.

Article Snippet: Do not aliquot laminin to plastic tubes from the original glass vial. table 1 | Antibodies used for staining cells to verify their identity. antibody Isotype source cat. no. Dilution OCT4 Mouse IgG Santa Cruz sc-5279 1:1000 PAX6 Mouse IgG DSHB PAX6 1:5000 SOX1 Goat IgG R&D AF3366 1:1000 OLIG2 Goat IgG Santa Cruz SC-19969 1:500 NKX2.2 Mouse IgG DSHB 74.5A5 1:50 NG2 Mouse IgG BD Pharmingen 554275 1:400 PDGFRα* Rabbit IgG Santa Cruz SC-338 1:400 O4* Mouse IgM Chemicon MAB345 1:50 PDGFRα, platelet-derived growth factor receptor alpha.

Techniques: Suspension, Derivative Assay